Test Methods

In Vitro Test

In vitro models are more ethical, economical and efficient than in vivo approach for the toxicological pre-assessment of Chinese herbal medicines. Moreover, in order to cover the whole pregnant period, a suitable in vitro model should also be incorporated. With consideration of the varied toxic effects of different Chinese herbal medicines in developmental stages, embryonic stem cell test, whole embryo culture, and micromass, which cover different stages (pre-implantation stage, after implantation stage and fetal growth stage respectively) of embryo development, could be recommended for assessment of Chinese herbal medicines toxicity.